| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.147822 |
| Chromosome: | chromosome 3 |
| Location: | 2882425 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g162750 | (1 of 2) K13207 - CUG-BP- and ETR3-like factor (CUGBP, BRUNOL, CELF) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGGCACACCTGTTTGTACGATGCAGAC |
| Internal bar code: | AGATTGGGTGAAACAACGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 647 |
| LEAP-Seq percent confirming: | 98.75 |
| LEAP-Seq n confirming: | 553 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTATACGTCGATTTGGT |
| Suggested primer 2: | GAAACGACACGAGATGACGA |