| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.147832 |
| Chromosome: | chromosome 16 |
| Location: | 1394606 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652150 | FBP4 | (1 of 1) K01103 - 6-phosphofructo-2-kinase / fructose-2,6-biphosphatase 3 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTCAGTGCCCGAAGCACACACCCACCGC |
| Internal bar code: | GACGTTTTAGTCCTCTGTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 610 |
| LEAP-Seq percent confirming: | 99.3023 |
| LEAP-Seq n confirming: | 427 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTCAATGTGGGTTAATGC |
| Suggested primer 2: | ATGAACTCGTCCGGGTACTG |