Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.147837 |
Chromosome: | chromosome 16 |
Location: | 1511697 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g653100 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGAAACATGAGTACGCAAATACATGGG |
Internal bar code: | GCACCTTCTCTGTCATTCGACT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 386 |
LEAP-Seq percent confirming: | 96.1652 |
LEAP-Seq n confirming: | 652 |
LEAP-Seq n nonconfirming: | 26 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCCTGATACCCTTCCTTTC |
Suggested primer 2: | CGCTTCTGCTTGTGTGAAAG |