| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.147862 |
| Chromosome: | chromosome 16 |
| Location: | 2351172 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659700 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACCTCGAGCCACTTTGCGCTTGGGTTGT |
| Internal bar code: | TTTGCATGGTCGCCGGAGGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 717 |
| LEAP-Seq percent confirming: | 99.9222 |
| LEAP-Seq n confirming: | 6426 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCGTGAACTAAATGCCCT |
| Suggested primer 2: | GTGTCAGCTGTGAAGGTGGA |