| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.147876 |
| Chromosome: | chromosome 10 |
| Location: | 471063 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g421000 | (1 of 10) IPR000104//IPR001810 - Antifreeze protein, type I // F-box domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTAGGTCGGCTGCCCAGTCACCGTTGGCG |
| Internal bar code: | ATGTATGGGTTTGTAGATTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1187 |
| LEAP-Seq percent confirming: | 99.7788 |
| LEAP-Seq n confirming: | 2255 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGAGTCAGGCCACACGGT |
| Suggested primer 2: | ATCTCGAACCACAGCTCGTT |