Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.147882 |
Chromosome: | chromosome 6 |
Location: | 5228181 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g282050 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGACCTACTGCCCTAGTTGTTGGTTGTT |
Internal bar code: | GAATTTGTGGTACCTAAGAGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 800 |
LEAP-Seq percent confirming: | 93.8775 |
LEAP-Seq n confirming: | 1242 |
LEAP-Seq n nonconfirming: | 81 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGTAGGATGGAGCAGCGGTA |
Suggested primer 2: | CATGGTAATGGGGGTAGGTG |