| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.147883 |
| Chromosome: | chromosome 3 |
| Location: | 4802742 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g178650 | MCM6 | (1 of 1) K02542 - DNA replication licensing factor MCM6 (MCM6); Minichromosome maintenance protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGAGTGGCGGAGTAGTGAGCAAGGAGTT |
| Internal bar code: | TGTCGCCCTCAGGCGAGACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 758 |
| LEAP-Seq percent confirming: | 99.6055 |
| LEAP-Seq n confirming: | 1010 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTCTGTCGTCAAATCCTG |
| Suggested primer 2: | TATGCGACATTGCAAGAAGG |