| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.147897 |
| Chromosome: | chromosome 6 |
| Location: | 2780954 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g271100 | CDKG1 | Cyclin dependent kinase; (1 of 4) K02206 - cyclin-dependent kinase 2 (CDK2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACATTTTCGTGACCACTTTGGGTGCTCT |
| Internal bar code: | TGTGTGACGTATTCTATCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 471 |
| LEAP-Seq percent confirming: | 99.7495 |
| LEAP-Seq n confirming: | 3982 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGGAAAGGACACACAGG |
| Suggested primer 2: | GTTTTAGCGAGTAAACGCCG |