| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.147899 |
| Chromosome: | chromosome 6 |
| Location: | 8896551 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g311050 | (1 of 1) K00167 - 2-oxoisovalerate dehydrogenase E1 component beta subunit (BCKDHB, bkdA2) | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGGAGCGATGCCGGCGCGGCGGCGAGCT |
| Internal bar code: | ATCACAGAGAGGGGCGCAGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 607 |
| LEAP-Seq percent confirming: | 99.9461 |
| LEAP-Seq n confirming: | 1854 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCTTCAACGCAAAAGGCT |
| Suggested primer 2: | GTGTATGCCACACAGGATGC |