Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.147913 |
Chromosome: | chromosome_3 |
Location: | 213906 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g144344 | antisense | 3'UTR|outside_mRNA |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATCTGTCATGGCGCGGCGACCTCATTCTTG |
Internal bar code: | GCTGGCGTACAGACATAGAGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 748 |
LEAP-Seq percent confirming: | 99.8267 |
LEAP-Seq n confirming: | 576 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCAGTGTTCAGTACTGGGG |
Suggested primer 2: | GCTGGCTTGAGAGTGAAACC |