Insertion junction: LMJ.RY0402.147913_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.147913
Chromosome:chromosome_3
Location:213906
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre03.g144344 antisense 3'UTR|outside_mRNA

Insertion site details

Flanking sequence (orientation from cassette outwards):ATCTGTCATGGCGCGGCGACCTCATTCTTG
Internal bar code:GCTGGCGTACAGACATAGAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:748
LEAP-Seq percent confirming:99.8267
LEAP-Seq n confirming:576
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TGCAGTGTTCAGTACTGGGG
Suggested primer 2:GCTGGCTTGAGAGTGAAACC