| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.147921 |
| Chromosome: | chromosome 1 |
| Location: | 682823 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003550 | (1 of 2) PTHR12231//PTHR12231:SF112 - CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN // RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 14, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCGCACCCGGGCGCCCGCTGCAGGTGAT |
| Internal bar code: | TTACAACGACACGCGTTCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 258 |
| LEAP-Seq percent confirming: | 98.6395 |
| LEAP-Seq n confirming: | 145 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTAAGCAGGCAGGTCAG |
| Suggested primer 2: | GAGTGGTCGTCTCGAATGGT |