| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.147924 |
| Chromosome: | chromosome 10 |
| Location: | 1871490 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431550 | ADH6 | Alcohol dehydrogenase; (1 of 1) PTHR11695//PTHR11695:SF559 - ALCOHOL DEHYDROGENASE RELATED // GALACTITOL-1-PHOSPHATE 5-DEHYDROGENASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATAGGACCAGCGCGGTCATGGATTTTGGG |
| Internal bar code: | TTGATAAATCGGCTTGAGCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 109 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTAAGCAGGAAAGAGTGC |
| Suggested primer 2: | CAGAGGTTTGTGTGTGTGGG |