Insertion junction: LMJ.RY0402.147940_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.147940
Chromosome:chromosome_6
Location:1197568
Confidence (%):73
Locus disrupted Locus common name Defline Orientation Feature
Cre06.g257600 FA1 Flagellar Autonomy Protein antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):CTCATCGCAAGCGTGTTAGGTCCACCCCTC
Internal bar code:AAGCGCAGGGCCTAACGGTCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:152
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CTAGCACAAGAGGGACGGAG
Suggested primer 2:CAGCAATCTCTGCCCCTTAG