| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.148127 |
| Chromosome: | chromosome 1 |
| Location: | 695474 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003650 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCATTGGGCTGCATTGGGACTCGCAGC |
| Internal bar code: | CGCGACTGCGAGACAGGGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 725 |
| LEAP-Seq percent confirming: | 95.0893 |
| LEAP-Seq n confirming: | 2343 |
| LEAP-Seq n nonconfirming: | 121 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACTGCGGCCTTTCTGACT |
| Suggested primer 2: | CTCGCTCTCTACAGCACACG |