| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.148128 |
| Chromosome: | chromosome 17 |
| Location: | 3046883 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g720600 | CAV1 | (1 of 5) PTHR10037//PTHR10037:SF218 - VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM // SUBFAMILY NOT NAMED; Voltage-gated Ca2+ channel, alpha subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATTGGGGAGAGCGGGGAGAGCAATGTGC |
| Internal bar code: | CTCTATACGTCCTGTTTACTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1266 |
| LEAP-Seq percent confirming: | 99.7038 |
| LEAP-Seq n confirming: | 1010 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGGGTTTACACTTCGCAT |
| Suggested primer 2: | ACGACCTCAGTCCACAGCTT |