Insertion junction: LMJ.RY0402.148143_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.148143
Chromosome:chromosome 16
Location:7007478
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g677765 (1 of 4) PF00207 - Alpha-2-macroglobulin family (A2M) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGATTTTTAAGCGCCGTGCCGCCCAGCGCC
Internal bar code:TGGAACTTCGGGGTCACTGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:788
LEAP-Seq percent confirming:99.7761
LEAP-Seq n confirming:1337
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GTTTGTGTCAATTGCGCATC
Suggested primer 2:TGACGTGAGCCATACGAAAG