| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.148143 |
| Chromosome: | chromosome 16 |
| Location: | 7007510 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677765 | (1 of 4) PF00207 - Alpha-2-macroglobulin family (A2M) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAAAATCAAAAGTCGGGGTTGGAGGAGT |
| Internal bar code: | GGGGGTCAAACGAAGAAGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 395 |
| LEAP-Seq percent confirming: | 58.6437 |
| LEAP-Seq n confirming: | 1496 |
| LEAP-Seq n nonconfirming: | 1055 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACGTGAGCCATACGAAAG |
| Suggested primer 2: | GTTTGTGTCAATTGCGCATC |