Insertion junction: LMJ.RY0402.148153_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.148153
Chromosome:chromosome 11
Location:3421065
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre11.g480800 (1 of 1) K14773 - U3 small nucleolar RNA-associated protein 23 (UTP23) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACATGGGGGAATGCTTGAGGTCTGGTCA
Internal bar code:GACATTAATAGGGCAGTCGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2
LEAP-Seq percent confirming:13.9385
LEAP-Seq n confirming:281
LEAP-Seq n nonconfirming:1735
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGGTGTCCAAACACGAGG
Suggested primer 2:ACCAACAAAAGGATACCCCC