Insertion junction: LMJ.RY0402.148167_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.148167
Chromosome:chromosome 3
Location:2142757
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g157200 (1 of 18) 2.7.10.2//2.7.11.1 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Non-specific serine/threonine protein kinase / Threonine-specific protein kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGAGAACGAGGGGGGTTACATTGATGATC
Internal bar code:TGTGCGAAGTACTTCACATACT

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:75.2475
LEAP-Seq n confirming:76
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGTAACCTGCATGTTTCCCT
Suggested primer 2:AACAATCTTTAGAGCGCGGA