| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.148168 |
| Chromosome: | chromosome 13 |
| Location: | 4366615 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g602850 | SRS8,SRP23,SRP33,SRS11 | Serine/arginine-rich pre-mRNA splicing factor; (1 of 1) K12897 - transformer-2 protein (TRA2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGGAATGAGGGACGACACGCCCCAGTGC |
| Internal bar code: | ACCCTGGGAAGTCGTTTCTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 241 |
| LEAP-Seq percent confirming: | 99.7695 |
| LEAP-Seq n confirming: | 5627 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCATACGCAACCCTTACC |
| Suggested primer 2: | ATCACAATTGGCGGGTAGAG |