| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.148169 |
| Chromosome: | chromosome 9 |
| Location: | 6443393 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407120 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCACAGTAACCACGTCGCCCCTTGGACA |
| Internal bar code: | TTTGATTCTGCGTCAAAGCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 643 |
| LEAP-Seq percent confirming: | 99.7101 |
| LEAP-Seq n confirming: | 3095 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAGGTATCGGCACCATTGC |
| Suggested primer 2: | CTCACAGCAGTAGCAGCAGG |