| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.148176 |
| Chromosome: | chromosome 17 |
| Location: | 166719 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g697334 | (1 of 10) PF13519 - von Willebrand factor type A domain (VWA_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGACATGTGTGAACACGCTGTTGCCTCCC |
| Internal bar code: | GAAGTCTTCGGGTGGAGCTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 683 |
| LEAP-Seq percent confirming: | 99.7787 |
| LEAP-Seq n confirming: | 9918 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGTGTTCAAGAGCTGGAG |
| Suggested primer 2: | GGAAATTTTAGGCCCGTAGC |