| Insertion cassette: | CIB1 | 
| Side of cassette: | 5' | 
| Strand: | + | 
| Strain: | LMJ.RY0402.148203 | 
| Chromosome: | chromosome 8 | 
| Location: | 275623 | 
| Confidence (%): | 58 | 
| Locus systematic id | Locus common name | Defline | Feature | 
|---|---|---|---|
| Cre08.g358560 | (1 of 6) PTHR22870//PTHR22870:SF201 - REGULATOR OF CHROMOSOME CONDENSATION // SUBFAMILY NOT NAMED | intron | 
| Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGCACACGCTGCGGCGCGTCCTTTTCCT | 
| Internal bar code: | TCTTTTGACTTGCGCATGGCTC | 
| Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 502 | 
| LEAP-Seq percent confirming: | 100.0 | 
| LEAP-Seq n confirming: | 67 | 
| LEAP-Seq n nonconfirming: | 0 | 
| LEAP-Seq n unique pos: | 3 | 
| Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGGCAGCTACGTCAACTT | 
| Suggested primer 2: | GGGGCAGAATGAGTCCAATA |