| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.148206 |
| Chromosome: | chromosome 16 |
| Location: | 5330426 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682050 | (1 of 1) 3.6.1.15 - Nucleoside-triphosphate phosphatase / Nucleoside-triphosphatase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCCAACCCGCTCGCGATCTAGCCCACC |
| Internal bar code: | TTTTCCTTGTCTGTTTGACAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 352 |
| LEAP-Seq percent confirming: | 99.6734 |
| LEAP-Seq n confirming: | 1831 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCAAAGCTCCATTTTTC |
| Suggested primer 2: | CCAACTCTTCCACAATGGCT |