| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.148207 |
| Chromosome: | chromosome 16 |
| Location: | 1380896 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652050 | ZXE3 | (1 of 1) PTHR13789//PTHR13789:SF234 - MONOOXYGENASE // SUBFAMILY NOT NAMED; Putative zeaxanthin epoxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTACTTATATGTCCTCGACGCAGCCCGTG |
| Internal bar code: | CAGAGGTTACCTCCTCGCCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 944 |
| LEAP-Seq percent confirming: | 99.8923 |
| LEAP-Seq n confirming: | 4636 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGGCATTGTGTTTGAGTA |
| Suggested primer 2: | GACTGACGTGATGTGGGTTG |