| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.148210 |
| Chromosome: | chromosome 12 |
| Location: | 7922834 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552650 | PAP8 | (1 of 1) PTHR23092:SF19 - NUCLEOTIDYLTRANSFERASE; Class-II RNA nucleotidyl transferase 8 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCGTGACGTGCTCAGTGATAACAAATC |
| Internal bar code: | AGTGTTACTTCGGTACCATAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1184 |
| LEAP-Seq percent confirming: | 99.4983 |
| LEAP-Seq n confirming: | 595 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGTCTTAGCACAGGGCGT |
| Suggested primer 2: | CCAGCTCCACGAGAGTTAGG |