| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.148215 |
| Chromosome: | chromosome_16 |
| Location: | 1943999 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g656300 | DPR1 | Dihydrodipicolinate reductase | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGGCTGCACTGCGGCCCTGGCTGCTTACGC |
| Internal bar code: | GGACTCTGGTAAGGTGCGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 451 |
| LEAP-Seq percent confirming: | 99.9256 |
| LEAP-Seq n confirming: | 2685 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCTGCACCTCCTCTCACT |
| Suggested primer 2: | TCTTGTGTGAAAGCACAGGC |