Insertion junction: LMJ.RY0402.148221_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.148221
Chromosome:chromosome 7
Location:1979362
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g325756 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTACGATAGTCCTAGAAATGACCAGGCCCA
Internal bar code:GGCCGATTTCCGGGGCACCTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:320
LEAP-Seq percent confirming:99.7675
LEAP-Seq n confirming:2575
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACGAGAGGCAGTTGCTTTGT
Suggested primer 2:CAATACGTGAGGTGCCAGTG