| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.148222 |
| Chromosome: | chromosome 11 |
| Location: | 2052679 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468600 | Hypothetical protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCATGCTAGACAATGAGCACGGTAGCAC |
| Internal bar code: | CGCGCTGGATCTGGAGTCTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 640 |
| LEAP-Seq percent confirming: | 99.8222 |
| LEAP-Seq n confirming: | 5054 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGGCTGAATGTCCTATGC |
| Suggested primer 2: | GTGCTTAATTGCTAAGCGCC |