| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.148236 |
| Chromosome: | chromosome 3 |
| Location: | 8716912 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204577 | DNJ31 | (1 of 1) IPR000104//IPR001623//IPR003072 - Antifreeze protein, type I // DnaJ domain // Orphan nuclear receptor, NOR1 type; DnaJ-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACATCATGCCTGCCGGTTCTCCACTAGCA |
| Internal bar code: | GTATCGACCGTTAAATGTAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 161 |
| LEAP-Seq percent confirming: | 77.5274 |
| LEAP-Seq n confirming: | 1204 |
| LEAP-Seq n nonconfirming: | 349 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGTGCTTGAAAGAACGCA |
| Suggested primer 2: | GTTCTCCAGGATCATGGGCT |