| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.148264 |
| Chromosome: | chromosome 7 |
| Location: | 4945279 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346550 | (1 of 8) 2.7.11.1//2.7.12.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Dual-specificity kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTTAGTGGCATGCAGCGTGCTGCACAGC |
| Internal bar code: | ATTGGAAGCCGCGATAGGAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 578 |
| LEAP-Seq percent confirming: | 99.8141 |
| LEAP-Seq n confirming: | 537 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGAGCATCTCCCACAGA |
| Suggested primer 2: | TCTTTCTTCGTCTTGCCGTT |