| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.148265 |
| Chromosome: | chromosome 16 |
| Location: | 5035965 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684550 | HAR2 | Possible 3-hydroxyacid dehydrogenase; (1 of 3) 1.1.1.26 - Glyoxylate reductase / NADH-dependent glyoxylate reductase | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGTGTAGACGCCGCGGCGATCCGCTCCG |
| Internal bar code: | TCGGTCGGCGAGCGCTGCGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 881 |
| LEAP-Seq percent confirming: | 99.8845 |
| LEAP-Seq n confirming: | 865 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTTTGCAAGAAATGCTGC |
| Suggested primer 2: | CAGTTGATGTCGCTGGCTAA |