| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.148266 |
| Chromosome: | chromosome 12 |
| Location: | 7486237 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556000 | ECP88 | 88 kDa extracellular polypeptide; (1 of 5) PF06742//PF06863 - Protein of unknown function (DUF1214) (DUF1214) // Protein of unknown function (DUF1254) (DUF1254) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCAGCAGTACGTCAGGGTGGCGGTTGG |
| Internal bar code: | TAGTGTTTAGGTCATACAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 887 |
| LEAP-Seq percent confirming: | 99.7859 |
| LEAP-Seq n confirming: | 2330 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTCCTACGGTGTGGATTA |
| Suggested primer 2: | AATGTAGGTGTCAGCCAGGG |