Insertion junction: LMJ.RY0402.148267_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.148267
Chromosome:chromosome 16
Location:4658001
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g687400 (1 of 18) K08824 - cyclin-dependent kinase-like [EC:2.7.11.22] (CDKL) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGCAGAGAACATGGACCCTTGAATCTAC
Internal bar code:TTGGCGGCGTGGTGACTTAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:560
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:938
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGGCAGTGACGAAGCTTATG
Suggested primer 2:GCCTCGCTACTCACTTGTCC