| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.148278 |
| Chromosome: | chromosome 1 |
| Location: | 5118221 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035600 | MSC2 | Mechanosensitive ion channel; (1 of 3) PTHR30566 - YNAI-RELATED MECHANOSENSITIVE ION CHANNEL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGTACCTCCACGTCCTCACTCCCACTA |
| Internal bar code: | ATGTCACACCCTAAACTTGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 197 |
| LEAP-Seq percent confirming: | 99.6288 |
| LEAP-Seq n confirming: | 2684 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCATGAGCGTGTCTGACT |
| Suggested primer 2: | CAATAACGCCCAACTGGACT |