| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.148286 |
| Chromosome: | chromosome 10 |
| Location: | 6168901 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g464050 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTGGTGCGGGGCAGATGCAGGGCCGTG |
| Internal bar code: | CCGTGAGCGGAGCGGCCATTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 111 |
| LEAP-Seq percent confirming: | 85.5754 |
| LEAP-Seq n confirming: | 528 |
| LEAP-Seq n nonconfirming: | 89 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGAATGGGAAGGGATGCAG |
| Suggested primer 2: | GAACCCGATCGGTCTTTGTA |