Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.148315 |
Chromosome: | chromosome_9 |
Location: | 2240626 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g392800 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACTCGGCTCCGCCGCCACCTCGCTGGCCGC |
Internal bar code: | GGGTCGCTACTTTACCAGAAAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 86 |
LEAP-Seq percent confirming: | 86.7769 |
LEAP-Seq n confirming: | 105 |
LEAP-Seq n nonconfirming: | 16 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCACCTCTTCCTTCCCTCC |
Suggested primer 2: | CAGCTGCTGTCGTATGTCGT |