| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.148318 |
| Chromosome: | chromosome 16 |
| Location: | 7187702 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681578 | APC8 | (1 of 1) K03355 - anaphase-promoting complex subunit 8 (APC8, CDC23); Anaphase promoting complex subunit 8 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACAGGTGTCCATTAAGGGTTGCTGGCC |
| Internal bar code: | TATCGCCGTTGTATTGCTGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 78 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGCGCCTAATCAGATACG |
| Suggested primer 2: | GGCTTATGATGGGGTCTGAA |