| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.148325 |
| Chromosome: | chromosome 16 |
| Location: | 5029253 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684600 | SEC5 | (1 of 1) K17637 - exocyst complex component 2 (EXOC2, SEC5); Component of the Exocyst Complex | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGCATCCGGTCACGTTCCCTTATTGTC |
| Internal bar code: | GTGAAGATGACGGCAGGCCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 683 |
| LEAP-Seq percent confirming: | 98.7443 |
| LEAP-Seq n confirming: | 6055 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGGTTGTGATATTGCTCA |
| Suggested primer 2: | CGAGCGAAAGGAGCATAGAG |