| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.148336 |
| Chromosome: | chromosome 10 |
| Location: | 600764 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g421800 | (1 of 21) PF02469 - Fasciclin domain (Fasciclin) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCAAAATGCAAAGTATCAAATTGCATCA |
| Internal bar code: | AGGCGGTCGGTGCTGGAACCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 929 |
| LEAP-Seq percent confirming: | 99.8826 |
| LEAP-Seq n confirming: | 1702 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCTTTCAGCACCAGAAGG |
| Suggested primer 2: | TGTCGATAAAGTGCTCACGC |