| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.148350 |
| Chromosome: | chromosome_2 |
| Location: | 6103970 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g113200 | GLN1,NSG18 | Glutamine synthetase | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCTGGCCGGGCGCCCTATGCGCGTCAGAAA |
| Internal bar code: | GGCAACGGCGCTATGTGGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 177 |
| LEAP-Seq percent confirming: | 95.9184 |
| LEAP-Seq n confirming: | 141 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAGGAAAGATGGGGTGAC |
| Suggested primer 2: | AGTGCAAACCGTTCCCTATG |