Insertion junction: LMJ.RY0402.148351_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.148351
Chromosome:chromosome 5
Location:3030077
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g238600 PHC36 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 36 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGTGGGCCAAGGCTGGTGACAACACCCT
Internal bar code:TAGGTTCGCCTCATTCTACAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:752
LEAP-Seq percent confirming:99.6781
LEAP-Seq n confirming:8980
LEAP-Seq n nonconfirming:29
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CCACAGTGAGAGACAAGGCA
Suggested primer 2:GCTACTCGACCTTCGTCACC