Insertion junction: LMJ.RY0402.148360_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.148360
Chromosome:chromosome 2
Location:6496252
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g116400 FAP120,DII7 Flagellar Associated Protein 120; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCAAACCTCGTCAGGGGGAGTGACGCCTC
Internal bar code:GGAGCAGAAGGACCTTCCCGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:214
LEAP-Seq percent confirming:91.215
LEAP-Seq n confirming:1952
LEAP-Seq n nonconfirming:188
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGACGTCTTCAAAACGGG
Suggested primer 2:TTTGGTTTAGTTTGGGCTGG