| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.148360 |
| Chromosome: | chromosome_2 |
| Location: | 6496252 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g116400 | DII7,FAP120 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGCAAACCTCGTCAGGGGGAGTGACGCCTC |
| Internal bar code: | GGAGCAGAAGGACCTTCCCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 214 |
| LEAP-Seq percent confirming: | 91.215 |
| LEAP-Seq n confirming: | 1952 |
| LEAP-Seq n nonconfirming: | 188 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGACGTCTTCAAAACGGG |
| Suggested primer 2: | TTTGGTTTAGTTTGGGCTGG |