| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.148422 |
| Chromosome: | chromosome 2 |
| Location: | 4134420 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g097950 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCATAACGCATACTCATACAGATTCAAC |
| Internal bar code: | ACACCGTACACGGGATATAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 691 |
| LEAP-Seq percent confirming: | 93.5841 |
| LEAP-Seq n confirming: | 846 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGAAGGTCGTTGGTTGG |
| Suggested primer 2: | AGGTTGTCGAGGTCAAGGTG |