| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.148436 |
| Chromosome: | chromosome 16 |
| Location: | 3728485 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g689001 | (1 of 100) IPR001810 - F-box domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTCCGTTCTGCGCTAGCTTGTAGAAAAT |
| Internal bar code: | CTCCTAGGCGTGTCCCGATACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 236 |
| LEAP-Seq percent confirming: | 99.5003 |
| LEAP-Seq n confirming: | 1792 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACCGTACCATACCTGCT |
| Suggested primer 2: | TGTTTCAGGATGCCAAATGA |