Insertion junction: LMJ.RY0402.148452_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.148452
Chromosome:chromosome 13
Location:3982369
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g591000 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCCACGTGCGAACAGCCAGTGACGACAC
Internal bar code:AGTCTGGGGAACTGTCTCGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:182
LEAP-Seq percent confirming:79.661
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CACACACCTACCGTTTGTCG
Suggested primer 2:CAAGGAAAGTGTCACGAGCA