| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.148456 |
| Chromosome: | chromosome 13 |
| Location: | 1661152 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g573950 | ZRT3,CrZIP3 | (1 of 6) K14709 - solute carrier family 39 (zinc transporter), member 1/2/3 (SLC39A1_2_3, ZIP1_2_3); Zinc-nutrition responsive transporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACCGCCTGCGGGCCTCGGTGCCGAGCC |
| Internal bar code: | CCTCCGGTGCTCCACTCAATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 164 |
| LEAP-Seq percent confirming: | 99.7717 |
| LEAP-Seq n confirming: | 437 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATCTACAGCCTGACGTGC |
| Suggested primer 2: | CAATGGACAAACGGTCACTG |