| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.148459 |
| Chromosome: | chromosome 15 |
| Location: | 1348746 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g641950 | (1 of 1) PF14846 - Domain of unknown function (DUF4485) (DUF4485) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATTCTAATCTTTCCAACACGCGTAATCG |
| Internal bar code: | AATGCATATTTACTGTCGCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 383 |
| LEAP-Seq percent confirming: | 99.7248 |
| LEAP-Seq n confirming: | 17396 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTGCTGATGTTGTTGT |
| Suggested primer 2: | GCAACCAGGGTTTCTACCAA |