| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.148509 |
| Chromosome: | chromosome 16 |
| Location: | 4669510 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687350 | ACO3,ACX3 | (1 of 1) PTHR10909:SF11 - ACYL-COENZYME A OXIDASE-LIKE PROTEIN; Acyl-CoA oxidase/dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGTGGAGGTGAGGGGAGGGCTGCCGCGC |
| Internal bar code: | TACGCCGCGCACGACTGTCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 784 |
| LEAP-Seq percent confirming: | 99.6737 |
| LEAP-Seq n confirming: | 3971 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACAAAGGTCGTCAAGCA |
| Suggested primer 2: | CTGGTACAGGTTGGGCTTGT |