| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.148519 |
| Chromosome: | chromosome 12 |
| Location: | 3405135 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g496900 | (1 of 1) IPR002711//IPR003615//IPR004919 - HNH endonuclease // HNH nuclease // Domain of unknown function DUF262 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTACATGCAAACACGTGCTTGTAGGGCC |
| Internal bar code: | AAGGATCACCGGAGTAATGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 919 |
| LEAP-Seq percent confirming: | 99.7725 |
| LEAP-Seq n confirming: | 5263 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTAGTGCGTGCGTGTGTCT |
| Suggested primer 2: | TACCCCTGCTCTGCTGTTTT |